Rapid and sensitive real-time PCR-DNA based method to detect Arachis hypogea allergens in various food samples

Peanut allergy is life threatening health concern that caused by allergenic proteins termed as Arachis hypogea present in peanut. The most fatal allergic reaction to peanuts anaphylaxis harmful the individuals who are peanut allergens. Sometimes, traces of allergens contamination also occurred during food processing. Hence, it very much important detect allergen products and our proposed study was carried out (Arachis allergen) various processed unprocessed foods using real-time Polymerase Chain (real-time PCR) technique a rapid sensitive method. First, DNA extracted from samples commercially available extraction kit further quantity & quality were tested. observed for amplification test presence chosen (peanut positive samples. sensitivity specificity method assayed its limit detection which found be 0.01% obtained efficiency 101.2%. Spiking powder maize flour performed at different concentrations.